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KMID : 0363320180390010001
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Journal of Korean Oriental Internal Medicine
2018 Volume.39 No. 1 p.1 ~ p.8
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The Effect of Gamidohongsamul-tang (GDT) on the Gene Expression Levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC Cells
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Lim Hyun-Chan
Jeon Sang-Yoon
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Abstract
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Objectives: This study seeks to investigate the effects of Gamidohongsamul-tang (GDT) on the Gene expression levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC cells.
Methods: HUVEC cells were treated at a concentration of 1, 10, 100 (μg/ml) of Gamidohongsamul-tang (GDT). To measure the NOS, KLF2, ICAM-1 and VCAM-1 gene expression in HUVEC cells, the synthesized cDNA was subjected to polymerase chain reaction (PCR) and electrophoresis was performed to verify gene expression level.
Results: 1. GDT significantly increased eNOS and KLF2 gene expression. 2. GDT significantly reduced ICAM-1 and VCAM-1 gene expression.
Conclusions: These experiments suggest that Gamidohongsamul-tang (GDT) regulates gene expression related with anti-dyslipidemic effects in HUVEC cells. In order to clinically apply this to diseases related to dyslipidemia, such as cardiovascular disease, additional in vivo experiments are needed to verify the anti-dyslipidemic effects of GDT.
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KEYWORD
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Gamidohongsamul-tang, anti-dyslipidemic, HUVEC cell
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